The Amino Acid Sequence of β-Galactosidase
نویسندگان
چکیده
منابع مشابه
The Amino Acid Sequence of P-Galactosidase
The method of Bruton and Hartley for the isolation of short NH2-terminal sequences of peptides has been applied to /3-galactosidase. Maleyl-P-galactosidase was hydrolyzed with thermolysin and the digest was acidified. After passage through Dowex 50, Thr-Met was obtained from the eluate. This procedure has been extended. For the isolation of a larger NH&erminal peptide, maleyl-protein was treate...
متن کاملAmino acid sequence of beta-galactosidase. V. Isolation and sequences of chymotryptic peptides.
The amino acid sequence of 72 chymotryptic peptides isolated from 14C-, 3H-labeled carboxymethyl-beta-galactosidase has been determined. A variety of techniques were used in the isolation procedures including separation by solubility, size, and ion exchange and paper chromatography. These peptides contain approximatley 500 amino acids, range in size from 2 to 26 residues, and give overlaps with...
متن کاملThe amino acid sequence of an extracellular nuclease of Staphylococcus aureus. 3. Complete amino acid sequence.
In a previous paper data were presented that permitted the linear arrangement of five fragments produced by cleavage of an extracellular nuclease of Staphylococcus aureus with cyanogen bromide (1). The amino acid sequences of the tryptic peptides prepared from these fragments, together with the partial sequences of chymotryptic peptides isolated from digests of the intact nuclease, have also be...
متن کاملOptimization of β-galactosidase production from lactic acid bacteria
β-galactosidase, commonly known as lactase, represents commercially important enzyme that is prevalently used for lactose hydrolysis in milk and whey. To the date, it has been isolated from various sources. In this study different strains of lactic acid bacteria were assessed for their β-galactosidase productivity, and Lactobacillus acidophilus ATCC 4356 resulted with the highest production pot...
متن کاملGalactosylation of caffeic acid by an engineered β-galactosidase.
Glycosylation is useful for improving the chemical properties and physiological functions of biologically and pharmacologically important compounds. The glycosylation of phenolic compounds can increase their solubility and stability in water. The addition of galactose residue has special meaning as it facilitates targeted delivery of drugs to the liver cancer cells with abundant galactose accep...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)81123-5